ProSci Antibodies Doing their part for Breakthrough Brain Mapping Technology
When Connectomics scientists set out to find a better way map neural connections with nanoscale precision, they faced an obstacle: no existing antibodies for some of their protein tags
So they turned to ProSci.
In a groundbreaking preprint on bioRxiv—“Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context”—researchers from E11 Bio unveiled PRISM, a new platform that combines protein barcodes, tissue expansion, and iterative immunostaining to reconstruct neurons with extraordinary accuracy.
To make it possible, they needed custom antibodies that didn’t yet exist.
ProSci was honored to be able to create them.
Enabling Discovery When No Antibody Exists
The PRISM method uses engineered “barcode” proteins to label individual neurons, allowing computers to trace entire networks with molecular context. Each barcode must be detectable with an antibody that’s:
Highly specific to its target
Stable through multiple rounds of staining and stripping
Free from cross-reactivity with dozens of other epitopes
For the PRISM team, three key tags for the protein bits—MOON, SUN, and TAG-100—had no commercially available antibodies.
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